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Date: 04 May 2024

despite a strong field, karasuno high volleyball team advances past preliminary round of harutaka tournament in miyagi prefecture to reach the third round as a fervent fan of the haikyu anime and manga series, i approached the much-anticipated haikyu movie with a mixture of excitement and apprehension however, my concerns were quickly dispelled as the film unfolded, delivering an exhilarating experience that surpassed all expectations the animation quality of the haikyu movie is nothing short of spectacular the dynamic and fluid animation sequences capture the intensity of the volleyball matches with a level of detail that is truly impressive the characters' movements, facial expressions, and the sheer energy on the court are brought to life in a way that enhances the overall viewing experience the animation team deserves commendation for their meticulous attention to detail, creating a visually stunning masterpiece the filmmakers skillfully balance character development, intense match sequences, and moments of humor, creating a well-rounded and captivating storyline the haikyu movie pays homage to the beloved characters that fans have grown to adore the film not only brings back the familiar faces of hinata, kageyama, and the rest of the team but also introduces new and compelling characters the movie delves deeper into the personal journeys of the players, making them even more relatable and endearing the soundtrack complements the movie's energy perfectly

the uplifting and heart-pounding music enhances the emotional impact of pivotal moments, creating a sensory experience that resonates with the audience in summary, the haikyu movie is a triumphant celebration of the beloved series it successfully captures the essence of the anime and manga while delivering a visually stunning and emotionally resonant experience whether you're a long-time fan or a newcomer to the world of haikyu, this movie is a must-watch for anyone seeking a thrilling and heartwarming sports anime adventure top picks sign in to rate and watchlist for personalized recommendations contribute to this page suggest an edit or add missing content when justin kuritzkes began writing what later became the hottest love triangle of the year, there was no star power involved he really talked about the brutality of sports, and the toll that it takes on the body and the mind and spirit he was very honest about his feelings of falling out of love with the sport and then falling back in love and having a relationship that was unhealthy for him then, very directly, something that inspired the movie from that book is that when agassi was really plunging down in the rankings, his coach, brad gilbert — who ended up being our tennis consultant on the movie — entered into a challenger event in nevada that was pretty rare for a guy who had won a couple grand slams and had been no that was really inspiring to me when i was thinking about the situation i could put the character art into at the beginning of the film

what made you want to write a tennis movie in the first place? i just happened to be watching the u open in it was the match between naomi osaka and serena williams in the final, and it was very controversial because serena williams got penalized for receiving coaching from the sidelines beyond sports? something that was going on with you guys personally? how would you have that conversation? and how could you communicate the tension of that situation silently using the language of film? but i started to really fall deep into an obsession that was how i started thinking about the movie, and that was really the spirit that guided me in writing it luca guadagnino said that he asked you to amp up the love triangle what did that aspect of the film look like before and after you started collaborating with him? the love triangle was always there, and the intertwining of their lives and their relationships and their desires was always the white sex com that made the movie go one of the first conversations we about the script was that luca felt it was very important that, in any love triangle, all the corners touch when i first heard that, i sort of felt that they already did that had not been in the script, a literal moment where they all come together finding the right place for it, so that it felt earned and organic and like it was always there in the first place there are going to be so many interpretations of that scene whether intentionally or not, you are intimately involved with somebody else

tennis is about being all alone, and being at a distance from somebody, and trying not to touch them trying to just miss them, and trying to trick them trying to make them think the ball is gonna go one place, and then go another place so tennis, of its nature, is erotic, and you usually play tennis against somebody of the same gender so tennis, by its nature, then becomes almost homoerotic was it always a story about a black woman and two white men? yeah, absolutely it was also, again, a movie that was partially inspired by me watching this match between these two very different black women at different stages in their careers that has everything to do with them being at these three different points in the american system not just what the sport means to them, but the precarity of their position in the sport to begin with and so much of what they find alluring about her has everything to do with that because so much of this film is about what goes unspoken, a lot is riding on the performances did you have anyone particular in mind while writing the screenplay? that would have been really crazy, for me to do that

but definitely once i finished the script and was thinking about who could do it, these three were right at the top of my mind we met on zoom after amy pascal sent it to her, and it was clear to me within two minutes of talking to her that she just understood tashi so completely, that even though they were very different people, she could see a way in, and that was incredibly exciting for me something that we talked about in that first conversation that i think is really, really interesting about her with this role is that the place that somebody like zendaya occupies our culture, in our world, is very much the space that tashi was supposed to occupy that was the kind of life that tashi was supposed to have, and then that gets taken away, and she had to figure out another way to get there, or she has to be at peace with not getting there it was really interesting for me to think about somebody like zendaya touching a character like that, because she just naturally brings so much knowledge to it, and so much of it is grounded in reality because she so intimately understands that dilemma it really felt like it was meant to be that then she ended up doing this to help keep your account secure, please log-in again for assistance, contact your corporate administratoroffering exclusive content not available on pornhub we update our porn videos daily to ensure you always get the best quality sex movieswhite sex com warning: either you have javascript disabled or your browser does not support javascript to view the video, this page requires javascript to be enabled

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enjoy the largest amateur porn community on the net as well as full-length scenes from the top xxx studios a text message with your code has been sent to: an email with the verification code has been sent to: use the 6 digit code sent to your two-factor authentication app the cute maid was fucked to orgasm by her master's cock, and creampie penetrated her pink pussy meehutao brazilian amateur couple has extreme orgasm together fadynha in the meantime, to ensure continued support, we are displaying the site without styles and javascript subcutaneous white adipose tissue scwat is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training ext and sex on its molecular landscape is not fully established specifically, the scwat of sedentary females displays -omic signatures related to insulin signaling and adipogenesis, whereas the scwat of sedentary males is enriched in terms related to aerobic metabolism these sex-specific -omic signatures are preserved or amplified with ext overall, this study underscores the powerful impact of sex on adipose tissue biology and provides a rich resource to investigate the scwat response to ext scwat is a dynamic storage and secretory organ composed of lipid-storing adipocytes and numerous other cell types for example, immune cells, endothelial cells and mesenchymal cells 123 through the release of a diverse array of signaling molecules such as adipokines, cytokines, growth factors and lipid-derived compounds, scwat impacts multiple biological processes that are critical for maintaining systemic health 12456 indeed, numerous factors secreted by or residing in scwat are implicated in the development of lifestyle-related diseases such as obesity, type 2 diabetes, insulin resistance and cardiovascular disease

accordingly, regulation of scwat biology, particularly in response to physiological stressors such as exercise, diet and age, is an important area for study ext improves scwat metabolic flexibility, lipid flux, insulin sensitivity and immune-cell polarization and expansion factors that are linked to the risk and severity of cardiometabolic diseases 24789 in humans and rodents, wat distribution and content varies considerably between sexes, possibly contributing to sexual dimorphism in disease risk 91011 although wat is one of the most sexually dimorphic tissues 1314few studies account for sex when studying exercise adaptations while many sex-driven differences may be attributable to sex hormones 915161718scwat is also sexually distinct before puberty, which suggests other potential contributors 91015 despite these interesting and translationally relevant differences in male and female wat, the molecular hubs orchestrating sexual dimorphism in wat and its response to ext remain largely unexplored to provide insight into potential molecular transducers of exercise within and across tissues, the molecular transducers of physical activity consortium motrpac recently published a multi-omic analysis of 18 solid tissues and blood from male and female fischer f rats that underwent progressive endurance ext notably, this work white sex com a strong sex-specific response to exercise in multiple tissues, especially in scwat leveraging this dataset, here we perform a deep dive into the sex-specific and temporal multi-omic response of scwat to 1, 2, 4 or 8 weeks of treadmill ext in male and female rats using this model, we identify profound differences in male and female scwat both at rest and in response to training and identify candidate molecular and cellular transducers that drive distinct phenotypes by sex and in the dynamic response to exercise further, our manuscript showcases methods to leverage the first publicly available motrpac multi-omics dataset to drive future hypothesis-based research this workload was selected for its translational relevance to human studies 19 sedentary control sed rats were age- and sex-matched with the 8-week-trained group vo 2 max and nuclear magnetic resonance nmr -derived body composition were measured at baseline and the start of the final training week in sed, 4- and 8-week-trained rats in animals selected for multi-omic analyses, vo 2 max relative to lean or total body mass increased similarly in both sexes following 8 weeks of ext, whereas vo 2 max relative to total body mass decreased significantly in sed rats after 8 weeks of inactivity fig body mass and whole-body fat decreased in male rats after 8 weeks of ext fig

in females, fat mass decreased after 4 weeks of training but returned to pretraining levels at 8 weeks fig over the same time period in sed rats, both males and females increased fat mass fig therefore, 8 weeks of ext attenuated increases in fat mass in females and reduced fat mass in males f male and female rats underwent a progressive treadmill training protocol tissues were collected from animals that completed 1, 2, 4 or 8 weeks of training as well as sed vo 2 max and nmr body composition analysis was performed as indicated each arrow in bc represents a different rat and they span from pre- to post-training values the rats within each group are arranged in ascending order by their pre-training measure tissue sections from each rat were automatically quantified using cellprofiler, with ten fields captured per section for analyses the confidence intervals for insulin are just for consistency; time point was not a significant predictor, so timewise comparisons were not performed further aligning with changes in fat mass in 8-week-trained females, adipocyte size distribution patterns were similar to those of sed controls fig in contrast, scwat from 8-week-trained male rats showed a significantly higher percentage of small adipocytes and a significantly lower proportion of larger adipocytes compared to sed fig

to further investigate sexual dimorphism in the ext response, we measured levels of common training-responsive clinical analytes in plasma supplementary table 1c—e in contrast, none of these variables were affected by ext in male rats overall, the results highlight sexual dimorphism in systemic and scwat-specific physiological adaptations to training, several of which serve as indices of enhanced lipid mobilization in males and retention of lipids in adipose stores in females sexual dimorphism was also apparent with regard to plasma glucose, glucagon and insulin levels fig despite sexual dimorphism in adipose-associated phenotypes, both sexes displayed similar metabolic adaptations in the skeletal muscle such as hexokinase 2 hk2 protein abundance extended data fig we first investigated molecules and pathways that were differentially expressed between sed males and females at the proteomic level, top differential proteins in males were related to lipid metabolism acot1, hadh and acbd7cholesterol transport npc2 and ketogenesis hmgcl fig the antioxidant sod1 was elevated in males relative to females at both the messenger rna and protein levels fig go terms related to oxidative metabolism, mitochondrial complex assembly, branched-chain amino acid bcaa catabolism, proteasome activation and ribosome subunits were consistently enriched in males at both the transcriptomic and proteomic levels fig in females, terms including lamellipodium and white sex com filament bundles were enriched in both -omes fig when probing the top proteomic networks displaying sexually distinct enrichments, sed males showed enrichment of terms related to aerobic metabolism, multivesicular trafficking including vesicle ubiquitination-dependent catabolism and sorting and translational regulation fig some of these metabolomic differences are consistent with enrichment of mitochondrial and amino acid metabolic pathways at the transcriptomic and proteomic levels in males

taken together, we observed notable differences in the scwat of sed male and female f rats at the transcriptomic, proteomic and metabolomic levels, providing a foundation for understanding the sexually dimorphic response of scwat to ext next, sample-level datasets were analyzed to identify differentially expressed features at each training time point by sex for example, 1-week-trained females versus sed females; fig in general, female scwat had a similar number of differential -omic features at all four ext time points, whereas the number of differentially expressed features increased with training duration in males fig the greatest number of differential transcripts were observed in 1- and 8-week-trained females and transcripts, respectively and 2-week-trained males transcripts fig six transcripts displayed differential expression at all training time points in females notably, grb14a negative modulator of insulin signaling 23was downregulated with ext, whereas olaha medium-chain mc fa thioesterase and hmgcs2a ketogenic enzyme with lipogenic roles 24were increased at all training time points in females the only transcript that displayed differential expression at all training time points in males was the hypoxia-inducible carbonic anhydrase ca12 25which was downregulated both males and females displayed an early 1 week adaptive immune response that was attenuated at later time points in females but remained positively enriched in males throughout ext extended data fig in 8-week-trained rats, terms related to the adaptive immune system, immune receptor activity, azurophil granules and vacuolar lumen were enriched in males, whereas transcription and translation regulatory processes, aerobic respiration and azurophil granules were enriched in 8-week-trained females fig in males, 18 proteins were differentially abundant at all time points, most of which were related to mitochondrial function maip, atp6v0d1 and immtmitochondrial solute transport slc25a11, slc25a3 and slc25a15 and vesicle transport tmed2 and sec61a1 lrpprc, a protein that regulates transcription of mitochondrial genes to promote lipid oxidation 26increased throughout training in males slc25a15, a mitochondrial ornithine carrier that promotes arginine synthesis, was also elevated throughout training in male scwat

arginine promotes lipolysis partially through ampk activation males also displayed enrichment of terms related to vesicle transport and ribosome activity and biogenesis throughout training fig females displayed differential abundance of five proteins at all four time points consistent with transcriptomic profiling, the insulin signaling repressor grb14 ref further, 4 or 8 weeks of training in females increased expression of anti-inflammatory and insulin-sensitizing orm1 ref both sexes displayed enrichment of terms related to transcription and translation at 8 weeks, with a more robust proteomic than transcriptomic response in males fig several hundred protein phosphorylation sites were differentially regulated with ext, with sexually dimorphic temporal patterns similar to proteomic ext responses supplementary table 4c in general, males displayed a more robust phosphoproteomic response following 4 and 8 weeks of ext fig given the well-defined role of mtorc1 in anabolic signaling, including in adipose tissue 31coupled with the observed downregulation of proteins and phosphoproteins associated with insulin signaling repression for example grb14 and camk2b in female scwat, we sought to characterize the phosphoproteins indicative of enriched mtor activity in adipocytes, acly promotes glut4 expression and de novo lipogenesis in a manner that is more pronounced in females this included non-targeted methods, as well as quantitative targeted methods focused on groups of chemically related analytes such as amino acids, acylcarnitines and nucleotides overall, the scwat metabolome displayed the least sexually dimorphic ext response, with the most differentially regulated metabolites appearing at 2 and 8 weeks in both sexes 2 weeks, and differential metabolites in females and males, respectively; 8 weeks, and differential metabolites fig the 4-week-trained time point displayed the most sexual dimorphism with differentially regulated metabolites in males and only 20 in females females displayed a prolonged early enrichment of pc and o-pc phospholipid species, along with a negative enrichment of sphingomyelin sm and unsaturated fas at 1 and 2 weeks males additionally displayed an increase in phosphatidylethanolamine pe at 4 weeks that subsided by week 8 fig

pe and pc are the major phospholipid species present in the phospholipid monolayer of the lipid droplet ld this interpretation is also consistent with the surge in mono- and di-nucleotide phosphate species and reduction in nucleotide triphosphates and increased amp:atp ratio corresponding to scwat fatty acyl-coa abundance at 8 weeks in males extended data fig